Fig. 7. Effect of TSP1 on mRNA expression of VEGF (A), FGF2 (B) and Caspase 3 (C) in in-vitro luteal cell culture. Briefly, cells isolated from late stage CL (Days 11-16) were stimulated with increasing concentrations of TSP1 (0, 50, 100, and 500ng/ ml) at 75-80% confluency over a period of 24, 48 and 72 h (n = 3/condition). Cells lysate was used for the extraction of total RNA and cDNA preparation. Two-way ANOVA with Tukey's HSD was performed to find the effect of concentration and time on the mRNA expression of VEGF, FGF2 & Caspase. Each point in the line chart indicates Mean ± SEM. The different superscripts denote statistical significance (p<0.05). Abbreviations: TSP1, Thrombospondin1; mRNA, Messenger RNA; VEGF, Vascular Endothelial Growth Factor, FGF, Follicular Growth Factor.